Liquid Chromatography

Liquid Chromatography

 

We use an offline Dionex HPLC system for high pH RP to fractionate the digested peptides prior to mass spectrometry. The buffer formula are as below:

  • Prepare 500mL of buffer A: Add 0.125mL of Ammonium Hydroxide in 500mL of analytical grade water. Fill the reagent vial with buffer A.
  • Prepare 250mL of buffer B: Add 2.5 mL of Ammonium Hydroxide in 250 mL of Acetonitrile.
  • buffer C: water.
  • Buffer D: Acetonitrile

Replace the water (analytical grade) for the pump.